Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration.

Cofactor regeneration – an important aspect of biocatalysis

Biocatalysis has made tremendous advances in the field of synthesis of industrially important products and intermediates. Cofactors are an important part of many enzymes which are involved in biocatalysis. These cofactors are expensive and stoichiometric additions are not economically feasible. This necessitates the in situ cofactor regeneration in biocatalytic processes. Various methods of reg...

Regeneration of cofactors for use in biocatalysis.

Cofactor-dependent enzymes catalyze many synthetically useful reactions. The high cost of cofactors, however, necessitates in situ cofactor regeneration for preparative applications. After two decades of research, several cofactors can now be effectively regenerated using enzyme or whole-cell based methods. Significant advances have been made in this area in the past three years and include the...

Regeneration of Cofactors for Enzyme Biocatalysis

Biomimetic NAD(+) models for tandem cofactor regeneration, horse liver alcohol dehydrogenase recognition of 1,4-NADH derivatives, and chiral synthesis.

The biocatalysis discipline has the potential to generate important chiral organic compounds by the use of enzymes, usually in the presence of critical co-factors. Therefore, practical methods for the regeneration of the co-enzyme, 1,4-NADH, have continued to be of significance in the biocatalysis field. In this vain, a variety of transition metal hydrides have been evaluated as catalysts for t...

Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration.

NAD(P)H-dependent oxidoreductases are valuable tools for synthesis of chiral compounds. The expense of the cofactors, however, requires in situ cofactor regeneration for preparative applications. We have attempted to develop an enzymatic system based on phosphite dehydrogenase (PTDH) from Pseudomonas stutzeri to regenerate the reduced nicotinamide cofactors NADH and NADPH. Here we report the us...